Journal: The Science of the Total Environment

Article Title: Sampling methods and assays applied in SARS-CoV-2 exposure assessment

doi: 10.1016/j.scitotenv.2021.145903

Figure Lengend Snippet: Data obtained from the chosen articles.

Article Snippet: , 43. Aerosol and surface contamination of SARS-CoV-2 observed in quarantine and isolation care , USA , No , Surface and air samples from COVID-19 patient rooms , Air sampling: Sartorius Airport MD8 air sampler operating at 50 Lpm for 15 min. Surface samples: sterile swabs , Viral RNA Extractions: using a Qiagen DSP Virus Spin Kit. RT-qPCR: using Invitrogen Superscript III Platinum One-Step Quantitative RT-qPCR System. Primers and probe used target the E gene of SARS-CoV-2. , • We detected viral contamination among all samples. , ( ) .

Techniques: Sampling, Lysis, RNA Extraction, Environmental Monitoring, Virus, Multiplex Assay, Northern Blot, Marker, RNA Detection, Isolation, Membrane, Control, Environmental Sampling, Amplification, Transmission Assay, Aerosol, Diagnostic Assay, Infection, Sterility, Real-time Polymerase Chain Reaction, Nested PCR, Reverse Transcription, Extraction, Purification, Digital PCR, Preserving, Quantitative RT-PCR, cDNA Synthesis, Magnetic Beads, Incubation, Modification, One Step RT-PCR, Cell Culture, Sequencing

Journal: Molecular Genetics & Genomic Medicine

Article Title: Clinical application of single‐molecule optical mapping to a multigeneration FSHD1 pedigree

doi: 10.1002/mgg3.565

Figure Lengend Snippet: Molecular diagnosis of FSHD1. (a) Southern blot hybridization of selected family members with FSHD1. Membrane‐bound EcoRI/HindIII (E/H), EcoRI/HindIII/BlnI (E/H/B), and HindIII (H) digested genomic DNA from affected and normal family members were hybridized to p13E‐11 (4q35 and 10q26), 4qA, and 4qB labeled probes. 4qA (*) and 4qB (+) fragments. (b) FISH analysis of family member 28‐III (FSHD1). Green signals represent D4Z4 repeat numbers. (c) SMOM analysis of FSHD1 family members. BssSI maps (vertical green bars) of 4qA (purple) and 4qB (orange) alleles for family members 28‐III and 58‐IV (FSHD1) and 59‐IV (normal). The position and number ( n ) of D4Z4 repeats for each allele are indicated by horizontal purple and orange bars

Article Snippet: The WBCs were resuspended in cell suspension buffer, embedded into agarose plugs (CHEF Genomic DNA Plug Kit, Bio‐Rad).

Techniques: Biomarker Discovery, Southern Blot, Hybridization, Membrane, Labeling

Journal: Cell Death Discovery

Article Title: NTRK1 knockdown induces mouse cognitive impairment and hippocampal neuronal damage through mitophagy suppression via inactivating the AMPK/ULK1/FUNDC1 pathway

doi: 10.1038/s41420-023-01685-7

Figure Lengend Snippet: A , B DCFH-DA staining results suggestedthatNTRK1 knockdown enhanced ROS accumulation in mouse neurons. n = 3. C The ATP content analysis indicated that NTRK1 knockdown resulted in reduced ATP content in mouse neurons. n = 3. D , E The results of JC-1 staining indicated thatNTRK1 knockdown decreased the mitochondrial membrane potential in mouse neurons. n = 3. F , G The mRFP-eGFP-LC3 assay revealed that NTRK1 knockdown suppressed the autophagic flux in mouse neurons. n = 3. ** P < 0.01.

Article Snippet: Mitochondria were isolated from mouse hippocampus tissues and mouse hippocampal neurons using the Mitochondrial Isolation Kit (C3601, Beyotime, Shanghai, China) in accordance with the directions.

Techniques: Staining, Knockdown, Membrane

Journal: International Journal of Molecular Sciences

Article Title: Acute Increases in Intracellular Zinc Lead to an Increased Lysosomal and Mitochondrial Autophagy and Subsequent Cell Demise in Malignant Melanoma

doi: 10.3390/ijms22020667

Figure Lengend Snippet: Mitochondrial membrane potential (Δψm) changes, ATP production and mitophagy in explant human melanoma cultures with lower free zinc stores (M5), average free zinc stores (M10) and higher free zinc stores (M9) exposed to 0.5 μM zinc pyrithione during 72 h. Cells were exposed to external zinc pyrithione and ( A ) loss of Δψm measured by decreased red fluorescence of JC-1 was determined in at least 1000 cells visualized by fluorescence microscopy. Results represent means ± SD of at least three independent experiments. # p < 0.05 significantly lower compared to the beginning of treatment with one-way ANOVA test and Dunnett’s post-test for multiple comparisons. ( B ) ATP production was measured in cell lysates by ATP bioluminescent assay kit ( C ). Mitophagy-specific fluorescence (Mitophagy Detection Kit) in cells exposed to zinc pyrithione alone or together with autophagy inhibitor chloroquine was determined fluorimetrically. Results represent means ± SD of at least three independent experiments. * p < 0.05 significantly higher compared to the beginning of treatment, # p < 0.05 significantly lower compared to the beginning of treatment with one-way ANOVA test and Dunnett’s post-test for multiple comparisons.

Article Snippet: Control and zinc pyrithione-treated cells of explant human melanoma grown in 96-well plates with black bottom were washed with PBS and incubated in 100 nM Mitophagy Dye Solution (Mitophagy Detection Kit (Dojindo Laboratories, Kumamoto, Japan)) for 35 min. After removal of the culture medium and washing with PBS, mitophagy-specific fluorescence was evaluated using a cell scoring module of MetaXpress ® Image Acquisition and Analysis Software.

Techniques: Membrane, Fluorescence, Microscopy, ATP Bioluminescent Assay

Journal: Poultry science

Article Title: Functional analysis of key members affecting egg production in the transglutaminase gene family in chickens.

doi: 10.1016/j.psj.2025.104794

Figure Lengend Snippet: Fig. 8. TGM4 inhibits late apoptosis of granulosa cells. (A) Effect of TGM4 overexpression on the expression of apoptosis-related marker genes in granulosa cells. (B) Quantification of TUNEL positive cell rate. (C) TUNEL assay for granulosa cell apoptosis upon TGM4 overexpression. (D) Flow cytometry scatter plot. The X-axis and Y-axis typically represent different fluorescence intensities (such as FITC-A and PE-A). Q1, Q2, Q3, and Q4 represent the proportion of dead cells, middle and late apoptotic cells, living cells, and early apoptotic cells, respectively (E) Flow cytometry assay for granulosa cell apoptosis upon TGM4 overexpression. *P < 0.05, **P < 0.01.

Article Snippet: TdT-mediated dUTP nick-end labeling (TUNEL) assay The apoptosis rate of chicken granulosa cells was assessed using the Elabscience® One-step TUNEL In Situ Apoptosis Kit (Elabscience, Wuhan, China).

Techniques: Over Expression, Expressing, Marker, TUNEL Assay, Flow Cytometry, Fluorescence

Journal: Poultry science

Article Title: Functional analysis of key members affecting egg production in the transglutaminase gene family in chickens.

doi: 10.1016/j.psj.2025.104794

Figure Lengend Snippet: Fig. 10. Schematic diagram depicting TGM4-mediated regulation of ovarian granulosa cell proliferation, apoptosis, and steroid hormone synthesis through the activation of related genes. The chicken image is sourced from the BioRender online platform (https://app.biorender.com/).

Article Snippet: TdT-mediated dUTP nick-end labeling (TUNEL) assay The apoptosis rate of chicken granulosa cells was assessed using the Elabscience® One-step TUNEL In Situ Apoptosis Kit (Elabscience, Wuhan, China).

Techniques: Activation Assay

Journal: Scientific Reports

Article Title: 5T4-specific chimeric antigen receptor modification promotes the immune efficacy of cytokine-induced killer cells against nasopharyngeal carcinoma stem cell-like cells

doi: 10.1038/s41598-017-04756-9

Figure Lengend Snippet: 5T4-28Z CAR gene modification promotes the anti-NPC activity of CIK cells in a 5T4-dependent manner. Target cells consisted of 5T4 low-expressing S18 and S26 cells and their spheroid cells with an intermediate or high level of 5T4 expression. Effector cells included 5T4-28Z-CIK, 5T4-28Z-CIK (NKG2D-), C-28Z-CIK and NT-CIK cells. ( a–d ) Briefly, 1 × 10 4 target cells per well were co-cultured with different effector cells at E/T ratios of 10/1, 5/1 and 2.5/1 for 4 h. The cytotoxicity of effector cells was examined with LDH release assays. Values in the line graphs represent the mean ± SD of three parallel wells. ( e ) Then, 2 × 10 4 target cells per well were co-incubated with 1 × 10 5 effector cells per well for 24 h. Effector cells cultured alone in medium served as the negative control. IFN-γ production of the effector cells was assessed using ELISAs. Values in the histogram represent the mean ± SD of three parallel wells. ( f ) In addition, 2 × 10 5 target cells per well were co-cultured with 1 × 10 6 effector cells per well for 5 h in the presence of 1 × Protein Transport Inhibitor Cocktail and anti-human CD107α-APC antibody or IgG isotype control. Effector cells cultured alone served as the negative control. Degranulation of effector cells was evaluated using FACS. Values presented in the histogram represent the mean ± SD of triplicate samples. ( g ) S26 cells or S26 spheroids labelled with CM-Dil were co-cultured with 5T4-28Z-CIK cells and monitored using an inverted fluorescence microscope with climate control. Images captured intermittently are displayed. The results are representative of at least three independent experiments. * indicates p < 0.05 (one-way ANOVA).

Article Snippet: Next, 50 μl of supernatant per well was collected to measure LDH release using a cytotoxicity LDH Assay Kit-WST ® (Dojindo) according to the manufacturer’s specifications.

Techniques: Modification, Activity Assay, Expressing, Cell Culture, Incubation, Negative Control, Control, Fluorescence, Microscopy